DCP Digital Colony Picker

产品特性

High-Efficiency Intelligent Screening

AI-based image recognition (brightfield/fluorescence) enables automatic and precise targeting of desired chambers, and one-click export of target clones

Ultra-Low Consumable Usage

5,000+ single clones (equivalent to 50+ agar plates) can be recovered from each chip, with significant reduction in experimental costs

Cultivation in Independent Chambers

Cultivation of single cells in independent chambers culturing effectively minimizes interference from fast growing cells

Flexible Reagent Exchange

Streamlined reagent exchange within chambers enables a wide range of experimental designs

Intuitive Data Visualization and Traceability

Offers multiple data visualization options, field-of-view toggling capability, and high-magnification imaging to observe cultivation chambers

Reduced Contamination Risk

Non-contact colony recovery and water-in-oil microdroplet collection reduce the need for frequent consumable changes and minimize aerosol contamination

技术参数

Cell Type	Bacterial, archaeal, and fungal cells; expandable to microalgae, plant, animal, and human cells
Sample Volume Requirement	Sample volume ＞ 20 μL
Excitation Wavelength	Blue-green light with center wavelengths of 470 nm and 530 nm
Number of Microchambers on Chip	16,000 microchambers (expandable), pL-volume for each microchamber
Colony Identifcation Approach	Al-assisted image recognition (brightfield / fluorescence)
Colony Identifcation Speed	1,000 single colonies per hour
Collection Plate	96-well microtiter plates for PCR or cultivation
Dimensions (W × D × H)	900 × 535 × 425 mm

应用案例

High-throughput screening of lactic acid-producing strains

Fluorescence intensity from a product-specific fluorescent sensor was used as the sorting criterion for lactic acid yield. In a sample containing approximately 1% target cells, a strain with a 17.6% higher lactic acid yield compared to the original strain was obtained after a single round of screening.

Screening of fast-growing strains

The colony area observed under bright-field microscopy was used as a metric for growth rate. After four rounds of screening under stress conditions, a fast-growing strain was obtained, with a specific growth rate 69.6% higher than that of the original strain.

Cultivation of low-abundance microorganisms

Starting from an environmental microbial sample, individual cells were cultivated in microchambers, leading to the successful isolation of multiple low-abundance strains. DCP reduced the cultivation time by two-thirds compared to conventional agar plate-based methods.

Microscopic de-duplication of clones

Environmental microbial community samples were directly introduced into an array chip. The detailed information on cell growth, development, and reproduction observed under the microscope was used to differentiate the strains. A single chip revealed seven distinct morphological types, and sequencing of the selected colonies confirmed that they represent seven different strains.

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数据软件

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