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Abstract :In CAST-R-HP, automated acquisition and machine learning of single-cell Raman spectra (SCRS) enable distinguishing individual H. pylori cells directly from a biopsy sample, with 98.5 ± 0.27% accuracy in ID. Moreover, by adding a 48- to72-h D2O feeding and drug exposure step prior to SCRS acquisition, CAST-R-HP reports AST for levofloxacin and clarithromycin with 100% accuracy, based on metabolic inhibition level.

SPECIES

Bacteria

Human

CAST-R DOI : 10.1093/clinchem/hvac082 PubMed : 35714147

2022.04.18 Pengfei Zhu, et. al.,   mLife   

Rapid, automated, and reliable antimicrobial susceptibility test from positive blood culture by CAST-R
AREA OF INTEREST Medicine

Abstract : Using the AST of tigecycline for sepsis as the main model, here we establish an automated system of Clinical Antimicrobials Susceptibility Test Ramanometry (CAST-R), based on D2O-probed Raman microspectroscopy. Featuring a liquid robot for sample pretreatment and a machine learning-based control scheme for data acquisition and quality control, the 3-h, automated CAST-R process accelerates AST by >10-fold, processes 96 paralleled antibiotic-exposure reactions, and produces high-quality Raman spectra.

SPECIES

Bacteria

CAST-R, Chips, Kits DOI : 10.1002/mlf2.12019 PubMed :

2022.02.23 Fan Li, et al.,   Front Cell Infect Microbiol   

Assessing efficacy of clinical disinfectants for pathogenic fungi by Single-cell Raman Microspectroscopy
AREA OF INTEREST Medicine

Abstract : Ramanome-based technique can catch pathogens (Candida ablicans as a model organism for fugal pathogens) that are still present and metabolically active in a tooth about to undergo root canal treatment even after they appear to have stopped growing. The technique could limit re-infections while reducing the amount of disinfectant required, in turn dialling down the chance of side-effects.

SPECIES

Fungi

CAST-R, Kits DOI : 10.3389/fcimb.2022.772378 PubMed : 35281452

2022.01.17 Yuhan Liu, et al.,   J Raman Spectrosc   

Single-cell Raman microspectroscopy-based assessment of three intracanal disinfectants’ effect on Enterococcus faecalis
AREA OF INTEREST Medicine

Abstract : Ramanome-based technique can catch pathogens (Enterococcus faecalis as a model organism for bacterial pathogens) that are still present and metabolically active in a tooth about to undergo root canal treatment even after they appear to have stopped growing. The technique could limit re-infections while reducing the amount of disinfectant required, in turn dialling down the chance of side-effects.

SPECIES

Bacteria

CAST-R, Chips, Kits DOI : doi.org/10.1002/jrs.6311 PubMed :

2021.08.31 Yuehui He, et al.,   mBio   

Intra-Ramanome Correlation Analysis unveils metabolite conversion network from an isogenic population of cells
AREA OF INTEREST Industrial Biotech

Abstract : Intra-Ramanome Correlation Analysis (IRCA) algorithm was introduced to construct a metabolite conversion network from a single instance of just one sample of isogenic cells.

SPECIES

Algae

RACS-Seq, Chips DOI : 10.1128/mBio.01470-21 PubMed : 34465024
Abstract : The Addressable Dynamic Droplet Array (aDDA) was invented for paralleled single-cell sample preparation and sequencing in a single, streamlined workflow, using yeast cells as an example. In aDDA, all the sample preparation steps, including single-cell isolation, cell lysis, amplification and product retrieval, are performed in sequence within one very small droplet.

SPECIES

Yeast

Kits DOI : 10.1002/smll.202100325 PubMed : 34296526

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