Abstract: The new technology, FISH-scRACS-seq (Fluorescence-In-Situ-Hybridization-guided Single-Cell Raman-activated Sorting and Sequencing), leverages the Raman-activated Optical Tweezers-based Cell Sorter developed by Single-Cell Biotech Inc. to combine species-targeting fluorescence in situ hybridization (FISH) with Raman spectroscopy, enabling the direct identification and isolation of functional single cells and their encoding enzymes from environmental samples. This dual-targeting approach enables researchers to match microbial species to their specific metabolic functions and uncover the underlying genomic resources that drive these processes. The method allows for the high-resolution study of ecological processes, linking species, metabolic abilities, genome sequences, metabolic pathways, and enzyme activities.

Abstract: The artificial intelligence-assisted Raman-activated cell sorting (AI-RACS) system automated the isolation and functional analysis of aluminum-tolerant microorganisms (ATMs) from acidic soil, marking a shift from manual, labor-intensive procedures to high-throughput automated workflows. By employing SCRS to assess cellular metabolic activity under aluminum stress, the researchers successfully identified and isolated 13 aluminum-tolerant strains, including Burkholderia spp., Rhodanobacter spp., and Staphylococcus aureus. These strains exhibited higher metabolic activity compared to those identified through traditional cultivation methods.

Abstract: Researchers employed metronidazole as a model antibiotic, and establish a method for eMIC-MA-based AST. The speed, accuracy, low cost, and mode-of-action revelation of CAST-R-HP for measuring metronidazole AST suggest it as a valuable tool for H. pylori eradication and resistance mechanism dissection.

Abstract: Researchers have introduced an innovative approach utilizing single-cell Raman spectroscopy (SCRS) technology. This method promises to revolutionize the yeast industry by offering a rapid, cost-effective, and precise method to evaluate yeast's metabolic activity and freeze resistance. The new SCRS method incorporates D2O probes to deliver accurate measurements of metabolic activity of baker's yeast cells, such as cell viability and overall cell vitality, at both population and single-cell levels. By utilizing fingerprint region of the Raman spectrum, this approach enables precise analysis of intracellular trehalose content, which is crucial for assessing cells’ freeze resistance. A significant advantage of this technology is its ability to detect viable but non-culturable (VBNC) cells, which are frequently overlooked by conventional methods.

Abstract: Researchers have developed a dual-modal (fluorescence/colorimetric) biosensor based on Fusarinine C (FsC) using the EasySort Compact, significantly enhancing the detection sensitivity and accuracy of Helicobacter pylori (H. pylori) in human fecal samples, achieving single-cell-level detection resolution.

Abstract: Leveraging EasySort, a novel magnetic nanoparticle-mediated technology for the enrichment, cultivation, and identification of functional microorganisms was developed, using the biological remediation of acetonitrile contamination as an example. This technology not only accelerates the domestication process but also overcomes the limitations of traditional separation and cultivation techniques. It enables the acquisition of microorganisms with specific functions (acetonitrile degradation) from activated sludge, enriches the resource library of acetonitrile-degrading functional bacteria, and provides a reference for the microbial remediation of acetonitrile pollution.