CAST-R Clinical Antimicrobial Susceptibility Test Ramanometry

CAST-R™ is an integrated instrument system that helps clinicians rapidly decide which (and at what level) antibiotic drug to prescribe. It includes two models, CAST-R™ Mini and CAST-R™ Pro.

Based on stable isotope-labeled Single-Cell Raman Spectroscopy, CAST-R™ allows direct identification of pathogen without cultivation, measurement of antibiotic resistance and its intercellular heterogeneity based on metabolic activity inhibition at single-cell resolution. As it is culture-free, CAST-R™ can provide results in as little as 3 hrs, i.e., a ten-fold acceleration from current methods.

Moreover, individual cells with specific drug resistance can be sorted using CAST-R™ Pro, supporting whole-genome sequencing to reveal genetic mutations that underlie the drug resistance. The genome of each drug-resistant cell can be amplified and sequenced with minimal bias and high-coverage.

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产品特性

Sample preparation in a high-throughput and automatic manner

For clinical applications, CAST-R™ introduces an automatic sample preparation module that minimizes manual manipulation and enables high-throughput sample preparation, drug co-incubation, sample cleaning, etc. At each time, CAST-R™ can analyze 24-48 samples, and the number of samples can be flexibly increased or reduced.

Rapid pathogen identification at single-cell resolution via SCRS

Based on a deep-learning algorithm and the Pathogen Single-Cell Raman Spectra (SCRS) Reference Database, SCRS from clinical samples are direclty analyzed for rapid identification. Currently, over 20 common bacteria and fungal pathogens can be identified, with 95% accuracy.

Rapid, label-free, phenotype-based AST at the single-cell level via MIC-MA

D₂O based single-cell Raman microspectroscopy can quantitatively probe bacterial response to drugs at single-cell resolution using the Raman shift at C-D band as a universal biomarker for metabolic activity. MIC-MA calculated from Raman spectra is superior to MIC as MIC-MA is able to detect the non-growing but metabolically active cells.

Target cell acquisition with in situ activity and amplification with high sequence coverage

In CAST-R™ Pro, the RAGE-Seq technology sorts the cell with targeted Raman signal in an aqueous phase, and then amplifies its DNA via a low-bias MDA reaction. The whole genome sequence coverage of a single E. coli cell can reach 99.5% (pL-level amplification in microdroplet), much higher than the control (23%; μL-level amplification).

技术参数

Precision	Single-cell level (cell culture free)
Clinical Sample	Urine / Blood / Sputum / Tissue fluid
Initial Input	Low to 10^4 CFU/mL
Accuracy	> 90% (identification time is less than 0.5 h)
AST Time	< 3 h
Cell Type	Bacterial, archaea, and fungal cells that > 0.5 μm (applicable to human, animal, plant and algae cells)
Mode of RACS	Liquid + micro-droplet (best way to keep cell activity, improve single-cell sequencing quality, and ensure the successful rate of single-cell culture)
RACS Working Environment	1-2 / min
Single-cell WGA	No contamination, low amplification bias and high sequence coverage
Data Type	Single-cell level: pathogen species, metabolic-based drug sensitivity phenotype, drug-resistance gene sequencing, genome variation, whole genome sequencing, cell morphology, and refractive index, etc.