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EasySort Lego Single-cell Microdroplet Sorting System

EasySort® Compact is a standalone system equipped with a large screen to visually control sorting. It achieves precise single-cell sorting, reliable encapsulation of target-cell into microdroplet and simple exportation of target one-cell microdroplet, while preserve the in situ state of the cell. EasySort is a complete yet simple solution for single-cell sorting in a "what-you-see-is-what-you-obtain" manner.
EasySort® can readily control the movement trajectory of single cells via optical tweezers, and rapidly wrap any target single cell (diameter > 1 μm) into a microdroplet by a gravity-driven technology, thus allowing downstream single-cell sequencing or cultivation. Therefore, EasySort® is universally applicable to any kinds of cells, including bacterial, archaeal, fungal, plant, animal and human cells.

The first commercialized instrument for precise sorting and then reliable micro-droplet packaging of single bacterial cell from complex samples, featuring preservation of cell viability, protection against contamination, and high quality of amplified single-cell DNA/RNA. 

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Features

The RAGE technology; 

Cells are sorted in an aquatic microenvironment liquid solution, to maximally retain the metabolic activity of single-cells.

Single-cell DNA/RNA extraction and amplification kits; 

Genome DNA amplification with high efficiency and low bias, with whole-genome sequence coverage exceeding 90%.

Flexibly coupled to bright field/fluorescence/Raman microscope; 

Served as single-cell sorting module for high-end microspectrometers and microscopes made by Thermo Fisher, HORIBA, and other leading precision instrument manufacturers.

Specifications

Type of cells Bacterial, fungal, microalgal, animal, plant and human cells (in liquid suspension)
Applications Ideal for environmental / clinical microbiome samples, and tissue samples from human / animal
Detection mode Bright field or fluorescence
Sorting mode RAGE
Throughput 1-2 cells / min (Auto / Manual)
Cell activity preservation Cells are sorted in an aquatic phase, to maximally retain the metabolic activity and physiological state of single-cells
Single-cell WGA No contamination, low amplification bias and high sequence coverage
Single-cell cultivation > 99% of sorted cells can be cultured successfully

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News & Press

EurekAlert!:Function-based sequencing technique permits analysis of just a single bacteria cell SCIENMAG:Function-based sequencing technique permits analysis of just a single bacteria cell PHYS:Function-based sequencing technique permits analysis of just a single bacteria cell

References

2021.06.29 Xiaoyan Jing,et al.,   mSystems

One-Cell Metabolic Phenotyping and Sequencing of Soil Microbiome by Raman-Activated Gravity-Driven Encapsulation (RAGE) AREA OF INTEREST Environment and Agriculture

SPECIES

Bacteria

Microbiome

RACS-Seq, EasySort, Chips, Kits DOI : 10.1128/mSystems.00181-21 PubMed : 34042466

SPECIES

Bacteria

Microbiome

CAST-R, RACS-Seq, EasySort, Chips, Kits DOI : 10.1002/smll.202001172 PubMed : 32519499
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